RNA Detection By Flow Cytometry

Invitrogen PrimeFlow RNA Assay expands the capability of flow cytometry to measure RNA. Combine PrimeFlow with immunolabeling of both cell-surface and intracellular proteins using fluorophore-conjugated antibodies. Researchers now can reveal the dynamics of RNA transcription together with protein expression patterns in single cell resolutions

Principle behind the PrimeFlow RNA assay

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1. Gene specific oligonucleotide target probe set binds to the target RNA sequence
2. Preamplifier and multiple amplifier molecules subsequently hybridize to target RNA for signal amplification
3. Label probe oligonucleotides conjugated to fluorescent dye detect up to four RNA targets per cell by flow cytometry